|용법:||Covid-19 RNA 추출||샘플:||구술인 Covid-19 또는 코 샘플|
|성분:||보호 솔루션, 반응 용액, 맥비드스 솔루션, 린스, 용리제||방법:||자기성 비드 방법|
Manual Extraction Nucleic Acid Extraction Kit ( Magnetic Bead Method )
Less Reagent Faster Operation Higher Accuracy
It is used for nucleic acids isolation, enrichment and purification.The processed products can be used for clinical in vitro diagnosis.
48 preps, 96 preps
|Component||48 preps||96 preps||Single Test Consumption|
|3ml||48||3ml||96||for nasal swab sample|
【Storage & Shelf Life 】
Applicable Samples: Whole blood, tissue homogenate, swab, serum, plasma, bronchoalveolar lavage fluid and other cell-free body fluids.
1) Manual operation: magnetic separator, centrifuge, dry bath.
2) Nucleic acid extraction system: automatic or semi-automatic nucleic acid extraction system based on magnetic bead absorption principle.
Things to do before starting: Mix all the reagents and gently invert 3-5 times before use. Shake the bottle containing Magbeads Suspension Solution and vortex for 2 minutes (before first use) or 1 minute (before subsequent use) to ensure that the magbeads are fully resuspended before use.
1.1 Break the testing swab into the tube with Preservation solution, seal the tube cover and heat it in 60°C for 20mins.
1.2 Take out the swab head, add 50μL Reaction solution and mix, then add 600μL Magbeads solution, seal the tube cover and gently mix it by mixer for 10-15mins in room temperature.
1.3 Use Magnet to attract the magbeads, pour the supernatant and leave the precipitation in the tube.
1.4 Add 600μL Rinse into tube to wash and mix for 5seconds, then use magnet to attract the magbeads again, repeat this operation for one time, then use vacuum pump to suck away the supernatant. Seal the tube cover and heat it in 100℃ for 1 min or wait till it is dry .
1.5 Add 30~50μL Eluent to wash the nucleic acid from magbeads, heat the tube in 60℃ to accelerate elution. Take the supernatant as template to PCR analysis.
Read from the extraction analyzer operation manual.